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Hybridization capture using short PCR products enriches small genomes by capturing flanking sequences (CapFlank)

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Tsangaras K., Wales N., Sicheritz-Pontén T., Rasmussen S., Michaux J., Ishida Y., Morand S., Kampmann M.L., Gilbert M.T.P., Greenwood A.
2014

Solution hybridization capture methods utilize biotinylated oligonucleotides as baits to enrich homologous sequences from next generation sequencing (NGS) libraries. Coupled with NGS, the method generates kilo to gigabases of high confidence consensus targeted sequence. However, in many experiments, a non-negligible fraction of the resulting sequence reads are not homologous to the bait. We ...

Méthodes de relevé; Organismes nuisibles des animaux

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