A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines

Bureau C., Lanau N., Ingouff M., Hassan B., Meunier A.C., Divol F., Sevilla R., Mieulet D., Diévart A., Périn C.
2018

Background: The clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and in vivo growth monitoring. Confocal microscopy coupled with the use of propidium iodide (PI) counter-staining is one of the most popular tools used to characterize the structure ...

Physiologie végétale : croissance et développement; Méthodes de recherche; Anatomie et morphologie des plantes