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CRISPR/Cas9-mediated efficient targeted mutagenesis has the potential to accelerate the domestication of #Coffea canephora#

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Breitler J.C., Dechamp E., Campa C., Rodrigues L.A.Z., Guyot R., Marraccini P., Etienne H.
2018

Genome editing, which is an unprecedented technological breakthrough, has provided a valuable means of creating targeted mutations in plant genomes. In this study, we developed a genomic web tool to identify all gRNA target sequences in the coffee genome, along with potential off-targets. In all, 8,145,748 CRISPR guides were identified in the draft genome of Coffea canephora corresponding to ...

Génétique et amélioration des plantes; Physiologie végétale : croissance et développement

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