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Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

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Fisher M.C., Ghosh P., Shelton J.M.G., Bates K.A., Brookes L., Wierzbicki C., Rosa G.M., Farrer R.A., Aanensen D.M., Alvarado-Rybak M., Bataille A., Berger L., Böll S., Bosch J., Clare F., Courtois E.A., Crottini A., Cunningham A.A., Doherty-Bone T.M., Gebresenbet F., Gower D.J., Höglund J., James T.Y., Jenkinson T.S., Kosch T.A., Lambertini C., Laurila A., Lin C.F., Loyau A., Martel A., Meurling S., Miaud C., Minting P., Ndriantsoa S., O'Hanlon S.J., Pasmans F., Rakotonanahary T., Rabemananjara F.C.E., Ribeiro L.P., Schmeller D.S., Schmidt B.R., Skerratt L.F., Smith F., Soto-Azat C., Tessa G., Toledo L.F., Valenzuela-Sánchez A., Verster R., Vörös J., Waldman B., Webb R.J., Weldon C., Wombwell E., Zamudio K.R., Longcore J.E., Garner T.W.J.
2018

Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and ...

Maladies des animaux; Ecologie aquatique; Méthodes de recherche

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