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Viral metagenomics approaches for high-resolution screening of multiplexed arthropod and plant viral communities

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François S., Filloux D., Fernandez E., Ogliastro M., Roumagnac P.
2018

Viral metagenomic approaches have become essential for culture-independent and sequence-independent viral detection and characterization. This chapter describes an accurate and efficient approach to (1) concentrate viral particles from arthropods and plants, (2) remove contaminating non-encapsidated nucleic acids, (3) extract and amplify both viral DNA and RNA, and (4) analyze high-throughput ...

Maladies des plantes

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