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Comparison of loop-mediated isothermal amplification, polymerase chain reaction, and selective isolation assays for detection of #Xanthomonas albilineans# from sugarcane

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Duarte Dias V., Fernandez E., Cunha M.G., Pieretti I., Hincapie M., Roumagnac P., Comstock J.C., Rott P.
2018

A loop-mediated isothermal amplification (LAMP) assay was developed and compared to polymerase chain reaction (PCR), nested PCR, and selective isolation assays for detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald. The pathogen was isolated on selective medium from 44 out of 45 (98%) samples taken from symptomatic stalks, and from 44 out of 70 (63%) samples from ...

Maladies des plantes

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