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Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry

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Rodrigues V., Baudier J.B., Chantal I.
2017

Quantifying cytokines is extremely important in studies of host¿pathogen interactions. Multiplex assays are commercially available but only for human and mouse cytokines. Here a method for the simultaneous quantification of five important bovine cytokines IFN?, IL-4, IL-10, IL-12, and TNF? in cell culture supernatants, using flow cytometry was reported. Functional beads from BD Biosciences ...

Maladies des animaux; Méthodes de relevé

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